SIMULATION-FIRST PROTOTYPE
Can we make replication visibly mechanistic?
Follow one DNA molecule as MCM loads, replication begins at selected sites, and forks move through S phase.
Replication simulation
Licensing
MCM double hexamers are loading
An illustrative molecule will begin automatically.
0loaded MCM
0origins fired
0%DNA copied
A new seeded realization is sampled for each molecule
Loaded MCMstationary double hexamer
Active CMGCMG at a moving fork
Replicated DNAtwo daughter fibers
Initiationloaded MCM becomes two moving CMGs
Terminationconverging forks complete intervening DNA
×Passive replicationincoming fork reaches loaded origin
Stalled forkstationary CMG at a fixed position
REPLICATION DETECTIVE
What do these symbols correspond to?
Use the live molecule and kymograph to test each interpretation independently.
REPLICATION SEQUENCE
From loading to completion
- 01LicenseMCM loads at multiple sites
- 02InitiateA subset becomes CMG
- 03ElongateBidirectional bubbles grow
- 04TerminateConverging forks finish the intervening DNA