PERTURB · OBSERVE · EXPLAIN
Control when DNA can replicate.
Shift licensing and initiation through one modeled cell cycle, change their strength, and watch a single molecule finish late, remain incomplete, or copy DNA more than once.
EXPERIMENTAL CONDITION
Regulatory controls
Regulated replication simulation
G1 licensing
MCM is loading before S phase
The reference condition begins automatically.
Simulated outcome
Running
0loaded MCM
0initiations
0%DNA copied
0%DNA re-copied
Sliders rerun one latent molecule; New samples another with four reference loading events and two reference initiations
Loaded MCMlicensed origin
Active CMGfirst-copy fork
Copied DNAfirst synthesis
Reloaded MCMloaded after local synthesis
Re-copied DNAcopy depth exceeds one
REGULATORY LOGIC
The handoff and replication feedback are distinct
- 01License in G1ORC competence and available CDT1 support MCM loading in this schematic
- 02Regulatory handoffS-CDK, DDK, and Geminin rise while ORC licensing competence falls
- 03InitiateS-CDK and DDK promote firing only at origins that loaded MCM
- 04Replication feedbackNascent DNA synthesis triggers replication-coupled CDT1 destruction
QUESTIONS
Explain what the perturbations reveal
Use the simulation to test each explanation. Each response is evaluated independently by an automated feedback service.
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